Coding

Part:BBa_M10011:Design

Designed by: Jennifer Brophy   Group: Berkeley BioE140L - S09   (2009-05-01)


{˂CPG_L6!}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needed to design appropriate linkers (to fuse the N and C termini of the native protien). Used 6aa linker (CDS: GGATCTAAGTCTCGTCGC) from PMID: 18480093.

The opening was created in loop 2 (aa 59-60) because the residues of the mature protein are presumably disordered because they are not visible in electron density maps.


Source

E. coli MG1655 genomic DNA

References